Part:BBa_K4761220:Design

adh6-linker1-ARO10

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2885
  Illegal SpeI site found at 1836
  Illegal PstI site found at 1714
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2885
  Illegal SpeI site found at 1836
  Illegal PstI site found at 1714
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2885
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2885
  Illegal SpeI site found at 1836
  Illegal PstI site found at 1714
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2885
  Illegal SpeI site found at 1836
  Illegal PstI site found at 1714
  Illegal NgoMIV site found at 991
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Stop codon of adh6 deleted for fusion expression.